Abstract

Purpose: Teratological capacities of lambda-cyhalothrin (LCH) and the rescuing abilities of vitamin E and olive oil in ovo exposure were analyzed in the golden black variety of domestic chicken. Methods: The study was conducted on fresh fertilized eggs collected and divided into 5 groups as follows: (1) Vehicle Control group (VCon) which received 0.1mL of 5% dimethyl sulfoxide (DMSO) solution in vegetable oil (2) the Lambdacyhalothrin group (LCH) was administered a 0.1mL of 5% DMSO and 0.01 mg/kg of LCH, all dissolved in vegetable oil. (3) Lambda-cyhalothrin+Vitamin E group (LCHE), was provided with a 0.1mL of 5% DMSO, 0.01mg/kg LCH & 0.1mg/kg of Vitamin E, dissolved in vegetable oil. (4) Lambda-cyhalothrin+ Vitamin E in Olive oil (LCHOE), received 0.1mL of 5% DMSO and 0.01 mg/kg LCH, both dissolved in 0.1mg/kg vitamin E solution (EVOO). Finally, the last group (5) Olive oil +Lambdacyhalothrin (LCHO) were administered 0.1mL of 5% DMSO, 0.01mg/kg LCH & 0.1mg/kg Vitamin E in EVOO. The embryos were removed from egg shells after 14th day of incubation, weighted and dissected for the removal of brains for histological outcomes. Results: There was significant decrease in embryonic weight, volume and density of brain in LCH group as compared to vehicle control. LCH treated embryos showed complete cell obliterations, microlesions and partial nuclear disintegration (micronuclear formation). Number of different types of neuronal cells (pyramidal, pyramidal-like, multipolar, local circuit) was also decreased in LCH treated embryos. However, vit E and olive oil treated embryos show rescuing effects towards all above mentioned abnormalities. Conclusions: Type II fluoridated pyrethroid LCH is highly toxic to developing chick brain which may unfortunately be present in chick feed. However, the precious ingredients of olive oil and vitamin E can reduce the risk of neurodevelopmental damages.