6th Edition of Neurology World Conference 2026

Abstract

Objective: The purpose of this study was to detect metabolites in hippocampal tissues of rats with KA-induced TLE based on 9.4T MRS imaging and analyse the changes of multiple metabolites in the hippocampus at different stages of epilepsy, finding biomarkers for the precise diagnosis and treatment of epilepsy.Methods: Injection of kainic acid（KA） into the amygdala was used to establish the epileptic rat model. Seven adult male SD rats were randomly included, and MRS imaging was scanned on the bilateral hippocampus of rats at 9.4T MRI. The scanning was performed on healthy stages and at 3, 8, and 29 days after the KA injection, respectively. A professional engineer analysed the MRS data and calculated the relative quantification of the metabolites. Finally, the statistical results were obtained by repeated measurement methods.Results:Among the 13 metabolites, there were statistically significant differences in the distribution of alanine (Ala)，Tau protein(Tau), Insulin+Glycine (total Ins+Gly) and glucose (Glc) in the hippocampus at different time points of disease. The distribution of phosphocreatine (PCr) and creatine (Cr) in the bilateral hippocampus showed significant differences.Conclusion: MRS Imaging technology based on high field intensity magnetic resonance can detect metabolites such as alanine, Tau protein, Insulin+Glycine, glucose, creatine phosphate, and creatine in rat hippocampus by non-invasive means, providing new biomarkers for the diagnosis of epilepsy, which will provide evidence for the application of MRS imaging in the accurate diagnosis and treatment of epilepsy.